IS2522-SGL Small Group Learning: Chromatography of Amino Acids
Amino acids are the fundamental building blocks of all proteins. Often times scientists may need to identify the amino acid contained in a protein. One such method for separating and identifying individual amino acids is paper chromatography. In this activity, students will perform a paper chromatography experiment on three known amino acids, and then use their results to identify the components of an unknown amino acid mixture. This kit contains enough material for 5 groups. Teacher’s Manual and Student Study Guide copymasters are included.
Aligned to the Next Generation Science Standards (NGSS)*
Disciplinary Core Ideas: PS1.A; PS1.B
Performance Expectations: MS-PS1-2; HS-PS1-3
Cross Cutting Concepts: Patterns
Engineering Practices: Planning and Carrying Out Investigations; Analyzing and Interpreting Data; Scientific Knowledge is Based on Empirical Evidence
0.5g Ninhydrin Powder
4 x 25mL 95% Ethanol Solution
2 x 25mL Chromatography Solvent
10mL Alanine Control
10mL Glycine Control
10mL Leucine Control
10mL Unknown Amino Acid Mixture
4 Capillary Tubes
1 Centrifuge Tube
7 Chromatography Sheets
Small quantity exemption 173.4
THIS PACKAGE CONFORMS TO 49 CFR 173.4 for domestic highway or rail transport only
*”Next Generation Science Standards” is a registered trademark of Achieve. Neither Achieve nor the lead states and partners that developed the Next Generation Science Standards was involved in the production of, and does not endorse, this product.
WARNING: This product can expose you to chemicals including Methanol/Methyl isobutyl ketone, which is known to the State of California to cause cancer and reproductive harm. For more information go to http://www.P65Warnings.ca.gov.